TargetPlex Focused Lung Cancer Panel and FFPE-Direct DNA Library Kit for Illumina NGS – COMBO – YST0095

TargetPlex Focused Lung Cancer Panel and FFPE-Direct DNA Library Kit for Illumina NGS – COMBO – YST0095 was designed with input from the European Cytopathology Consortium, this multiplex amplicon panel targets actionable mutation is Non-Small Cell Lung Cancer (NSCLC) for next-generation sequencing (NGS) sequencing from formalin-fixed paraffin-embedded (FFPE) samples directly from 5 – 15 um thick tissue sections on a glass slide.  This kit leverages the use of the proprietary FFPE-Direct Library Preparation workflow and the TargetPlex Noise Cancellation Technology, this rapid, addition-only, 3.0-hour workflow is ideal for the analysis of ~ 3100 actionable NSCLC mutations covering eight oncogenes (EGFR, KRAS, BRAF, NRAS, PIK3CA, PDGFRA, KIT, and ALK) without the need for separate, labor-intensive, and time-consuming, pre-analytical FFPE DNA extraction, purification, and isolation steps. The NSCLC two-pool design is comprised of only 26 primer pairs per primer pool, thus allowing for ultra-sensitive mutation detection. The proprietary primer modification and unique primer-pair tiling design of the NSCLC panel enable high detection sensitivity and high-target specificity.

This kit includes:

• Optimized primer pools (2) of 26 amplicons each
• HiFi PCR Master Mix for target enrichment
• 8 Illumina indexes
• TargetPlex proprietary primer digestion enzyme mix
• Magnetic beads for DNA clean-up and size selection
• Library amplification primers

Applications: Targeted DNA sequencing from FFPE for Illumina NGS platforms.

FOR RESEARCH USE ONLY.