Covid-19 RT-qPCR Detection Kit – YST00153

TaqMan RT-qPCR SARS-CoV-2 Detection Kit contains the assays and controls for a real-time reverse transcription polymerase chain reaction (RT-PCR) test intended for the qualitative detection of nucleic acid from SARS-CoV-2.
Principle: RT-PCR primers and three sets of fluorescent probes for amplicons were designed to cover simultaneously three gene regions: ORF 1ab, E and N (all FAM) for SARS-CoV-2. Additionally, human ACE2 (VIC) was used in the kit as an internal reference control.

RNA isolated and purified from upper and lower respiratory specimens is reverse transcribed to cDNA and subsequently amplified in the real-time PCR detection system. In the process, the probe anneals to a specific target sequence located between the forward and reverse primers. During the extension phase of the PCR cycle, the 5’ nuclease activity of Taq polymerase degrades the probe, causing the reporter dye to separate from the quencher dye, generating a fluorescent signal. With each cycle, additional reporter dye molecules are cleaved from their respective probes, increasing the fluorescence intensity. Fluorescence intensity is monitored at each PCR cycle by real-Time PCR System.